Proteomic Analysis of Human Endothelial Cells

Investigation of calcitonin receptor-like receptor properties in primary human dermal lymphatic endothelial cells.

Calcitonin receptor-like receptor (CLR) is a G protein-coupled receptor (GPCR) expressed in human skin and primary human dermal lymphatic endothelial cells (HDLEC). CLR-mediated signalling in the lymphatic system is implicated in skin-related diseases, including lymphoedema. However, protein binding partners defining pharmacological properties of CLR, beyond its agonists and receptor activity-modifying proteins (RAMPs), are insufficiently studied. The aim of this study was to identify proteins capable of binding CLR in HDLEC. During the project, a mass spectrometry (MS)-based label-free quantitative proteomics pipeline in conjunction University of Hull High Performance Computer (HPC) VIPER was developed using primary HDLEC. This work aims to provide novel biological insights in pharmacological properties of human CLR, with focus on proteome associated with CLR in HDLEC.